Journal: PLoS ONE

Article Title: Effects of Danshensu on Platelet Aggregation and Thrombosis: In Vivo Arteriovenous Shunt and Venous Thrombosis Models in Rats

doi: 10.1371/journal.pone.0110124

Figure Lengend Snippet: Chemical structure of danshensu (C 9 H 10 O 5 , molecular weight = 198.17).

Article Snippet: Danshensu (purity 98%) was provided by Nanjing Zelang Medical Biological Technology Co. Ltd (Nanjing, China).

Techniques: Molecular Weight

Journal: PLoS ONE

Article Title: Effects of Danshensu on Platelet Aggregation and Thrombosis: In Vivo Arteriovenous Shunt and Venous Thrombosis Models in Rats

doi: 10.1371/journal.pone.0110124

Figure Lengend Snippet: Blood was drawn 60 min after intragastric danshensu administration. Platelet aggregation was induced by diphosphate (ADP) and arachidonic acid (AA). Data are expressed as mean ± SEM (each group, n = 10). **P <0.01 with control group.

Article Snippet: Danshensu (purity 98%) was provided by Nanjing Zelang Medical Biological Technology Co. Ltd (Nanjing, China).

Techniques: Control

Journal: PLoS ONE

Article Title: Effects of Danshensu on Platelet Aggregation and Thrombosis: In Vivo Arteriovenous Shunt and Venous Thrombosis Models in Rats

doi: 10.1371/journal.pone.0110124

Figure Lengend Snippet: Blood was drawn 60 min after intragastric danshensu administration. Platelet aggregation was induced by arachidonic acid (AA). Data are expressed as mean ± SEM (each group, n = 10). The blood stasis model was built during the interval between when 2 injections of adrenaline hydrochloride (Adr) were given to rats placed in ice-cold water. ##P <0.01 compared with normal control. *P <0.05 **P <0.01 compared with Adr control.

Article Snippet: Danshensu (purity 98%) was provided by Nanjing Zelang Medical Biological Technology Co. Ltd (Nanjing, China).

Techniques: Control

Journal: PLoS ONE

Article Title: Effects of Danshensu on Platelet Aggregation and Thrombosis: In Vivo Arteriovenous Shunt and Venous Thrombosis Models in Rats

doi: 10.1371/journal.pone.0110124

Figure Lengend Snippet: Danshensu and aspirin (water, as a control) were incubated with COX-1 (A) or COX-2 (B) for 10 min and then AA was added. COX enzyme activities are reflected by the amount of PGE 2 produced. PGE 2 production was measured by ELISA. Enzyme activity in the sample without treatment (water alone) served as control and results are expressed as inhibitory rate of COX-1 and COX-2 activity compared with solvent control.

Article Snippet: Danshensu (purity 98%) was provided by Nanjing Zelang Medical Biological Technology Co. Ltd (Nanjing, China).

Techniques: Control, Incubation, Produced, Enzyme-linked Immunosorbent Assay, Activity Assay, Solvent

Journal: PLoS ONE

Article Title: Effects of Danshensu on Platelet Aggregation and Thrombosis: In Vivo Arteriovenous Shunt and Venous Thrombosis Models in Rats

doi: 10.1371/journal.pone.0110124

Figure Lengend Snippet: Rats were given 15, 30 and 60 mg/kg danshensu intragastrically and 20 mg/kg aspirin for 7 days. Venous thrombus formation was induced by inferior vena cava (IVC) ligation to produce thrombus after last administration.Vein walls were harvested from venous thrombosis and the expression of COX-1 and COX-2 was assessed by western analysis (A and C). β-actin was measured to confirm equal loading of proteins. Densitometric analysis of COX-1 (B) and COX-2 (D) expression is represented by the mean from 3 separate experiments. Data were normalized to β-actin levels. Thromb: thrombosis. ##P <0.01 compared with normal control. *P <0.05, **P <0.01 compared with thrombotic control group.

Article Snippet: Danshensu (purity 98%) was provided by Nanjing Zelang Medical Biological Technology Co. Ltd (Nanjing, China).

Techniques: Ligation, Expressing, Western Blot, Control

Journal: PLoS ONE

Article Title: Effects of Danshensu on Platelet Aggregation and Thrombosis: In Vivo Arteriovenous Shunt and Venous Thrombosis Models in Rats

doi: 10.1371/journal.pone.0110124

Figure Lengend Snippet: Effects of danshensu on plasma TXB 2 and 6-keto-PGF 1α levels and TXB 2 -6-keto-PGF 1α ratio in rats the venous thrombosis model.

Article Snippet: Danshensu (purity 98%) was provided by Nanjing Zelang Medical Biological Technology Co. Ltd (Nanjing, China).

Techniques: Clinical Proteomics

Journal: PLoS ONE

Article Title: Effects of Danshensu on Platelet Aggregation and Thrombosis: In Vivo Arteriovenous Shunt and Venous Thrombosis Models in Rats

doi: 10.1371/journal.pone.0110124

Figure Lengend Snippet: Effects of danshensu on plasma TXB 2 and 6-keto-PGF 1α levels and TXB 2 -6-keto-PGF 1α ratio in rats in the arteriovenous shunt model.

Article Snippet: Danshensu (purity 98%) was provided by Nanjing Zelang Medical Biological Technology Co. Ltd (Nanjing, China).

Techniques: Clinical Proteomics

Journal: PLoS ONE

Article Title: Effects of Danshensu on Platelet Aggregation and Thrombosis: In Vivo Arteriovenous Shunt and Venous Thrombosis Models in Rats

doi: 10.1371/journal.pone.0110124

Figure Lengend Snippet: The effect of danshensu on the hemorheological parameters in rats.

Article Snippet: Danshensu (purity 98%) was provided by Nanjing Zelang Medical Biological Technology Co. Ltd (Nanjing, China).

Techniques: Control

Journal: PLoS ONE

Article Title: Effects of Danshensu on Platelet Aggregation and Thrombosis: In Vivo Arteriovenous Shunt and Venous Thrombosis Models in Rats

doi: 10.1371/journal.pone.0110124

Figure Lengend Snippet: A: Photographs of gastric mucosa in the control group and danshensu, aspirin and aspirin + danshensu treatment groups are shown. B: The hemorrhagic ulcer index (mm 2 ) for each condition. Values are shown as the mean ± SEM(each group, n = 10). **P <0.01 compared with the control rats. ##P <0.01 compared with the aspirin-treated rats.

Article Snippet: Danshensu (purity 98%) was provided by Nanjing Zelang Medical Biological Technology Co. Ltd (Nanjing, China).

Techniques: Control